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The combined use of silica gel (SGA) and an anion-exchange resin (AER) with added silica gel as sedimentation/re-suspension media (S/R media) in the presence of selected polymers and salts was investigated. The results of tests performed on two genetic recombinant E. coli clones containing plasmids containing the neomycin phosphotransferase II (NPTII) gene (pKAS-2) and the phosphoglycerate kinase gene (PGK) (pKAS-3) and not containing any genetic marker, showed that S/R media with 10 mM citrate as an anion and 3-20% (w/v) SGA in the presence of 0.02% (w/v) AER, exhibited greater efficiency for the recovery of viable cells and plasmid DNA (pDNA) than plating (i.e. using only S/R media without AER). The growth and the plasmid yield in E. coli JM109 transformed with pKAS-2, pKAS-3 and pDNA were compared under different conditions. The plasmid yield was determined on agar
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